Anti-HAT1 Antibody

Histone Acetyltransferase 1 (HAT1) is a type B catalytic enzyme that acetylates newly synthesized histones H3 and H4 in the cytoplasm before chromatin deposition. The unconjugated rabbit polyclonal IgG reagent from Antibodies.com is supplied as 50 µl at approximately 1 mg/ml in PBS containing 0.1% sodium azide and 50% glycerol, and is recommended to be stored at -20 °C and protected from repeated freeze-thaw cycles to preserve activity. Affinity-purified to greater than 95% purity by SDS-PAGE, the polyclonal antibody was raised against recombinant full-length human HAT1 and detects endogenous levels of the ~49 kDa target protein. Validated workflows span western blot, immunohistochemistry, immunofluorescence, immunoprecipitation, and chromatin immunoprecipitation, providing flexibility across chromatin biology, DNA damage response, and replication-coupled histone modification studies. The reagent cross-reacts with human, mouse, and rat samples, supporting comparative epigenetics and gene expression experiments, while the unconjugated format lets researchers conjugate the IgG to fluorophores, enzymes, or beads depending on downstream detection needs. Packaged in a single-use vial, the antibody is intended for research use only and is used in investigations of nuclear import of newly synthesized histones and the coupling between acetylation and chromatin assembly.