Anti-HACL1 Antibody

HACL1 (2-hydroxyacyl-CoA lyase 1) is a peroxisomal enzyme that catalyzes the carbon-carbon bond cleavage of 2-hydroxyacyl-CoAs, generating formyl-CoA and the corresponding aldehyde during alpha-oxidation of branched-chain fatty acids. This rabbit polyclonal antibody from Antibodies.com is raised against the human HACL1 protein and is supplied as 100 µl of unconjugated antiserum validated for Western blot (WB) and immunohistochemistry (IHC) workflows on human samples.

The reagent detects endogenous human HACL1, supporting research into peroxisomal alpha-oxidation, branched-chain fatty acid catabolism, and disorders linked to impaired phytanic acid turnover such as Refsum disease. Because the antibody is unconjugated and polyclonal, it can be paired with a range of secondary antibodies and chromogenic or fluorescent detection chemistries, allowing the same primary reagent to be used across multiple assay platforms.

Recommended working dilutions, antigen retrieval conditions, and blocking strategies should be optimized empirically for each sample type, fixation method, and detection system. The antibody is intended for research use only and is not validated for diagnostic or therapeutic applications.