Anti-HA Tag Antibody [HA52]

This mouse monoclonal IgG3 antibody targets the hemagglutinin (HA) epitope tag, a nine-amino-acid sequence widely engineered into recombinant fusion proteins for detection and purification. Supplied by Antibodies.com as a lyophilised 100 µg preparation, clone HA52 binds the YPYDVPDYA motif with high specificity, enabling reliable identification of HA-tagged constructs across multiple experimental platforms.

The unconjugated format supports flexible pairing with secondary detection reagents chosen by the user, while the murine host origin simplifies routine laboratory workflows that rely on anti-mouse secondary antibodies. The IgG3 isotype and monoclonal clonality contribute to lot-to-lot consistency, an important consideration for reproducible data across long-running studies.

Validated applications include Western blot for assessing expression levels of tagged proteins, immunoprecipitation for isolating HA-tagged complexes from cell lysates, and immunostaining for localising tagged constructs in fixed samples. The reagent is shipped lyophilised and reconstituted with buffer before use, allowing extended shelf life without cold-chain degradation. Researchers working on tagged recombinant expression constructs, protein-protein interaction studies, and pull-down assays frequently select this reagent for its clean performance and reliable batch consistency.