Anti-GZMB Antibody

Granzyme B is a serine protease stored in the cytotoxic granules of activated CD8+ T lymphocytes and natural killer cells, where it triggers caspase-dependent apoptosis in target cells upon directed granule release.

This unconjugated rabbit polyclonal reagent from Antibodies.com targets the human GZMB protein for detection across western blot, immunohistochemistry, and immunofluorescence workflows. Investigators working on tumor-infiltrating lymphocyte biology, immune checkpoint inhibitor responses, viral clearance models, and graft-versus-host reactions use GZMB staining to map cytolytic activity in tissue sections and fixed cell preparations.

Each vial ships as a 100 µL liquid aliquot and should be stored frozen at -20 °C in single-use fractions to preserve activity across freeze-thaw cycles, with brief handling on ice for active aliquots. Supplied without a fluorophore or enzyme tag, the reagent supports custom detection panels—HRP-, biotin-, or fluorophore-conjugated secondaries can be paired to match the assay platform, whether chromogenic IHC of formalin-fixed paraffin-embedded sections, immunoblotting of whole-cell lysates, or IF imaging of permeabilized cells.