Anti-GTF2H3 Antibody

Raised in rabbit hosts against General Transcription Factor IIH subunit 3, this polyclonal antibody from Antibodies.com targets the p34 component of the TFIIH complex that participates in RNA polymerase II transcription initiation and the nucleotide excision repair pathway. Supplied as a 100 µl vial at 1 mg/ml in PBS with 0.1% sodium azide and 50% glycerol, the unconjugated preparation is validated for Western blot analysis, immunohistochemistry on paraffin and frozen sections, and immunofluorescence staining of cultured cells. Antibody reactivity has been confirmed against human, mouse, and rat samples, supporting comparative studies of DNA repair capacity across species and profiling of TFIIH subunit composition in tumor tissue and cell-line panels. The polyclonal host response provides recognition of multiple epitopes along the target, which is helpful when probing denatured lysates for the full-length GTF2H3 band and when localizing the protein in fixed tissue. Storage at -20 °C in the supplied glycerol buffer preserves activity through repeated freeze-thaw cycles; brief refrigeration at 4 °C is appropriate for working aliquots during active use. Researchers studying xeroderma pigmentosum, trichothiodystrophy, and transcription-coupled repair will find this reagent a focused tool for tracking GTF2H3 expression alongside its TFIIH partners.