Anti-GTF2F2 Antibody

Raised in rabbit, this polyclonal primary antibody from Antibodies.com targets GTF2F2 (also referred to as BTF4, RAP30, and TFIIF subunit beta), a general transcription factor component involved in RNA polymerase II–mediated transcription initiation. The reagent is supplied as an unconjugated IgG preparation at 1 mg/ml in a 50 µl vial, allowing flexible dilution across multiple experimental setups. It is validated for Western blotting, immunohistochemistry, and immunofluorescence applications, and it has been confirmed to react with human samples, making it suitable for studies in human cell lines and tissue specimens. The immunogen is a recombinant full-length human GTF2F2 protein, so the antiserum recognizes endogenous levels of the ~30 kDa target. Affinity purification using an epitope-specific immunogen yields a clean reagent with >95% purity by SDS-PAGE, and the antibody is formulated in PBS containing 0.1% sodium azide and 50% glycerol as a cryoprotectant. For short-term use the vial should be kept cold, and for continuous use it is recommended to store the product at –20 °C; freeze-thaw cycles should be minimized to preserve binding activity. Researchers working on transcription factor IIF biology, RNA polymerase II complex assembly, or chromatin-mediated transcriptional regulation will find this reagent a robust detection tool. As with all Antibodies.com primary antibodies, the reagent is intended for research use only and is not validated for diagnostic or therapeutic applications. The polyclonal format provides broader epitope coverage than a monoclonal clone, which can be advantageous when studying post-translational modifications or when working with samples where the target may be partially degraded.