Anti-GTBP Antibody

GTBP (G/T mismatch-binding protein), the product of the PMS2 gene, is a core component of the DNA mismatch repair (MMR) machinery that corrects base-pair mismatches and small insertion/deletion loops generated during replication. From Antibodies.com, this rabbit polyclonal reagent is raised against GTBP and supplied as 100 µl of unconjugated antiserum, supporting western blot, immunohistochemistry, immunofluorescence, and ELISA workflows with human, mouse, and monkey samples.

In molecular pathology and cancer research, PMS2 status is routinely evaluated alongside MLH1, MSH2, and MSH6 to determine microsatellite instability (MSI) and to screen Lynch syndrome. Loss of nuclear PMS2 staining by IHC is a clinically informative pattern in colorectal, endometrial, and ovarian tumors, and the reagent is used by labs building MMR-deficient panels or validating cell-line models.

For western blot, the antibody recognizes the PMS2 protein at approximately 96 kDa in lysates from MMR-proficient cells, and it pairs with MSH6 detection when characterizing MutSα complexes. The unconjugated format allows flexible secondary antibody choice for chromogenic IHC, fluorescent IF, or chemiluminescent WB detection, and the polyclonal backbone increases the chance of recognizing multiple epitopes on the target across different sample preparations.