Anti-GSTT1 Antibody

The GSTT1 enzyme (Glutathione S-Transferase Theta 1) plays a central role in cellular Phase II detoxification, catalyzing conjugation of reduced glutathione to electrophilic substrates including environmental toxins and byproducts of oxidative stress. This unconjugated rabbit polyclonal reagent from Antibodies.com is validated for Western blot and immunohistochemistry workflows and detects the target in human, mouse, and rat samples. The antibody is supplied as 100 µl of polyclonal, unconjugated liquid and is raised against antigens corresponding to the GSTT1 sequence. Endogenous GSTT1 migrates near 27 kDa on reduced SDS-PAGE, so blots are usually probed at working concentrations suited to chemiluminescent readout. Researchers commonly use this reagent to characterize GSTT1 expression in hepatic and extrahepatic tissues, to investigate the well-studied GSTT1 null deletion in epidemiological and pharmacogenetic studies, and to compare baseline versus induced transferase levels in toxicology and oxidative-stress models. The unconjugated format leaves the antibody compatible with any secondary reagent of the user's choice, including HRP-, biotin-, or fluorophore-conjugated secondaries.