Anti-GST Tag Antibody

The GST tag (glutathione S-transferase) is a 26 kDa fusion partner widely used in recombinant protein expression and purification workflows. This mouse polyclonal anti-GST tag antibody from Antibodies.com is raised against the tag sequence and binds the GST moiety in fusion constructs, enabling detection across multiple experimental readouts.

Validated applications include western blot, immunofluorescence, and immunoprecipitation, making it a versatile tool for confirming recombinant expression, localizing fusion proteins in fixed cells, and pulling down GST-tagged complexes from lysates. The unconjugated IgG preparation is supplied at 50 µL per vial, allowing flexible downstream conjugation or direct detection depending on the assay.

As a polyclonal reagent, this antibody recognizes multiple epitopes on the GST tag, which can improve signal strength in western blot and IP workflows relative to monoclonal alternatives. The mouse host species is compatible with commonly used anti-mouse secondary reagents for standard detection chemistries in research use only applications.

Store refrigerated at 4 °C for short-term use; for longer-term storage, aliquot the antibody and freeze to preserve activity across repeated freeze-thaw cycles. Researchers working on recombinant protein production, fusion construct validation, and pull-down studies will find this reagent useful for tracking GST-tagged proteins from initial expression through downstream functional analysis.