Anti-GST Antibody

The Antibodies.com anti-GST polyclonal antibody is raised in rabbit and specifically recognizes glutathione S-transferase, a 26 kDa affinity tag commonly fused to recombinant proteins for expression, detection, and purification studies. This unconjugated IgG reagent is supplied as a 100 ug liquid aliquot in a single vial and is validated for western blot detection of GST-tagged constructs as well as immunoprecipitation of GST fusion proteins from cell lysates. Researchers routinely apply this antibody to confirm in-frame expression of GST-tagged baits prior to affinity purification on glutathione resin or to track tagged proteins during pull-down and co-immunoprecipitation assays. The polyclonal format allows recognition of multiple epitopes on the GST moiety, improving the likelihood of detecting partially proteolyzed or truncated fusion partners. For best stability, store at -20 °C in working aliquots, avoid repeated freeze-thaw cycles, and centrifuge briefly before use; the reagent is intended for research use only and is compatible with standard chemiluminescent and enzyme-conjugated (HRP or AP) secondary detection workflows.