Anti-GST Antibody

The Anti-GST Antibody from Antibodies.com is a mouse monoclonal reagent of the IgG1 isotype, supplied as a 100 µg liquid formulation for detection of glutathione S-transferase fusion proteins. The GST tag, typically derived from Schistosoma japonicum, is a 26 kDa protein commonly fused to recombinant constructs to enable affinity purification on glutathione agarose and straightforward detection in immunoassays.

Validated in Western blot and ELISA workflows, this unconjugated primary antibody binds the GST moiety independently of the fusion partner, allowing researchers to confirm expression levels, screen expression clones, and normalize tagged-protein loading. Handle the vial by brief centrifugation before opening, store refrigerated for short-term use, and prepare working aliquots to limit freeze-thaw cycles; for longer storage keep frozen at -20°C or colder.

For Western blot, working concentrations of 0.5 to 2 µg/mL are typical when paired with anti-mouse IgG secondary conjugates; for ELISA, the antibody can be coated directly or used as a detector. As a research use only reagent, this antibody is suited to laboratories working with GST-tagged constructs in bacterial, yeast, and mammalian expression systems, and supports chemiluminescent or chromogenic readout depending on the chosen secondary.