Anti-GRWD1 Antibody

The Anti-GRWD1 polyclonal antibody from Antibodies.com is raised in rabbit hosts and recognizes the glutamate-rich WD repeat-containing protein 1 (GRWD1), a WD40 repeat histone chaperone implicated in ribosome biogenesis, DNA replication initiation, and nucleolar stress responses. This unconjugated IgG reagent is supplied as a liquid in a 100 µg vial and is validated across multiple immunoassays, including western blot, immunoprecipitation, chromatin immunoprecipitation (ChIP), and immunocytochemistry/immunofluorescence (ICC/IF), with reactivity confirmed against human, mouse, and rat samples.

Recommended working dilutions generally range from 1:500 to 1:2000 for western blot, while ChIP assays typically use 2 to 5 µg of antibody per immunoprecipitation reaction. Store the vial at -20°C in aliquots to limit freeze-thaw cycles and avoid prolonged light exposure; under these conditions the reagent remains stable for the shelf life indicated on the product label. Centrifuge briefly before opening and handle with nuclease-free tubes when used for chromatin applications.

GRWD1 has attracted research interest as a candidate biomarker in several malignancies, including colorectal, lung, and breast cancers, where elevated expression correlates with proliferation and prognosis. The reagent is therefore frequently used in studies of ribosome assembly factors, R-loop resolution, and p53-dependent stress signaling. Research use only.