Anti-GRK2 (T23) Antibody

Raised against the N-terminal region surrounding residue Thr23 of G protein-coupled receptor kinase 2 (GRK2, also known as ADRBK1 or BARK1), this rabbit polyclonal primary antibody from Antibodies.com is supplied as 50 µl of a 1 mg/ml stock in phosphate buffered saline at approximately pH 7.2 with 0.05% sodium azide as preservative. GRK2 phosphorylates activated GPCRs such as the β-adrenergic receptor to drive receptor desensitization and β-arrestin-mediated internalization, so the reagent is widely used to study GPCR regulation in cardiac, neuronal, and metabolic signaling research. Cross-reactivity with the mouse and rat orthologues is reported alongside reactivity with human GRK2, supporting side-by-side work in rodent disease models and human tissue specimens. Validated applications include western blot for monitoring GRK2 expression levels and phosphorylation-state shifts, plus immunohistochemistry on fixed sections for cell-type and subcellular localization studies. The unconjugated format leaves the antibody open to in-house HRP or fluorophore conjugation, fitting both standard chemiluminescent western blot workflows and custom immunostaining protocols. For routine use store refrigerated at 4 °C; for long-term storage, aliquot the 1 mg/ml stock and freeze at -20 °C, limit freeze-thaw cycles, and protect from light. The polyclonal nature of the reagent provides epitope-redundant recognition, which is helpful when probing GRK2 across tissue contexts where post-translational modification patterns may otherwise compromise detection.