Anti-GRB2 Antibody

GRB2 (Growth Factor Receptor Bound Protein 2) is an SH2/SH3-domain adaptor of roughly 25 kDa that links activated receptor tyrosine kinases such as EGFR and HER2 to the SOS-Ras-MAPK signaling axis, which is why it is routinely examined in growth-factor, proliferation, and tumor-biology work. The unconjugated rabbit polyclonal reagent from Antibodies.com is supplied as 100 µl of antiserum and has been validated for western blot and immunohistochemistry on human, mouse, and rat specimens, with reactivity confirmed across all three species rather than restricted to a single lineage.

The antibody is provided as a liquid aliquot that should be kept refrigerated and handled in single-use portions to preserve polyclonal titer across repeated freeze-thaw cycles; a band near 25 kDa is expected in whole-cell lysates from serum- or EGF-stimulated cultures, while immunohistochemistry on formalin-fixed sections typically yields cytoplasmic staining in receptor-tyrosine-kinase-driven tissues. Recommended starting dilutions commonly fall in the 1:500 to 1:2000 range for western blot and 1:100 to 1:500 for IHC, though each laboratory should titrate against its own positive and negative controls. For research use only; not intended for diagnostic or therapeutic applications.