Anti-GRB2 Antibody

GRB2 (Growth Factor Receptor Bound Protein 2) is a critical adaptor protein that bridges activated receptor tyrosine kinases to downstream Ras/MAPK signaling cascades, mediating key processes in cell proliferation, migration, and differentiation. This unconjugated mouse monoclonal antibody from Antibodies.com specifically detects the GRB2 protein in western blot applications, producing clear bands that allow researchers to monitor expression changes in signaling studies.

Validated for reactivity against human, mouse, rat, and monkey samples, the reagent is supplied as 100 µl of purified immunoglobulin in a stabilization buffer containing 0.1M Tris-Glycine (pH 7.4), 150 mM NaCl, 0.2% sodium azide, and 50% glycerol. The monoclonal format ensures batch-to-batch consistency, while the unconjugated presentation gives laboratories flexibility to pair the antibody with their preferred secondary detection reagents or custom labeling strategies.

Recommended applications include signal transduction research, investigation of receptor tyrosine kinase pathways, characterization of growth factor responses, and studies of oncogenic Ras signaling. Researchers working on EGF, FGF, PDGF, or insulin receptor pathways will find this reagent useful for tracking GRB2 phosphorylation status, membrane recruitment, or complex formation with binding partners such as SOS1, Shc, and dynamin.