Anti-GRAP Antibody

This rabbit polyclonal antibody from Antibodies.com recognizes GRAP (GRB2-related adaptor protein), an intracellular signalling molecule that contains SH2 and SH3 domains and functions as a molecular bridge between activated cell-surface receptors and downstream effector cascades. The unconjugated reagent is raised in rabbit and supplied as 100 µl of stock solution, sized for laboratories that run multiple western blot membranes, tissue sections, or fluorescence imaging experiments from a single aliquot.

Validated applications include Western blot (WB) for detection of GRAP in cell lysates and tissue homogenates, immunohistochemistry (IHC) for localization within fixed tissue sections, and immunofluorescence (IF) for subcellular distribution studies in cultured cells. Reactivity is reported for human GRAP, supporting research into receptor-proximal signalling events in lymphocytes and other cell types where this adaptor couples tyrosine-phosphorylated receptors to downstream pathways.

The polyclonal format recognizes multiple epitopes on the GRAP protein, an advantage when probing for splice variants, isoforms, or post-translationally modified forms of the adaptor. Researchers investigating T-cell receptor signalling, growth factor responses, or adaptor-mediated scaffolding complexes commonly use this reagent for confirmatory western blot bands, tissue-level expression mapping, and fluorescence-based colocalization studies.