Anti-Granzyme B Antibody

Manufactured by Antibodies.com, this rabbit polyclonal reagent is raised against Granzyme B and validated for use in western blotting (WB) and immunohistochemistry (IHC) workflows. The 50 µl unit is supplied as an unconjugated affinity-purified IgG at a concentration of 1.0 mg/mL, formulated in phosphate buffered saline (without Mg²⁺ and Ca²⁺) at pH 7.4 with 150 mM NaCl, 0.02% sodium azide, and 50% glycerol. It detects endogenous levels of total Granzyme B protein across human, mouse, and rat samples, making it a versatile choice for cross-species cytolytic granule studies. The immunogen is a synthesized peptide derived from the human Granzyme B sequence, and the antibody is shipped at 4 °C for short-term transit; upon receipt, it should be aliquoted and stored at −20 °C with freeze/thaw cycles avoided to preserve activity. Polyclonal clonality in a rabbit host provides broad epitope coverage, while the unconjugated format gives researchers full flexibility to pair the reagent with their preferred secondary detection system for chromogenic or fluorescent IHC, as well as standard WB membranes. Each lot is affinity-purified from rabbit antiserum by epitope-specific affinity chromatography, helping to minimize off-target binding in demanding applications such as paraffin-embedded tissue staining and whole-cell lysate blotting. For laboratory research use only, not intended for diagnostic or therapeutic applications.