Anti-GR (Ab-226) Antibody

This polyclonal antibody from Antibodies.com specifically recognizes the glucocorticoid receptor (GR), a ligand-activated nuclear receptor that mediates corticosteroid hormone signaling and downstream transcriptional responses in human cells. Raised in rabbit hosts, the reagent is supplied as 100 µl of unconjugated antiserum and is validated for western blot (WB) and immunohistochemistry (IHC) workflows on human-derived samples.

The unconjugated format gives laboratories flexibility to pair the reagent with their preferred detection chemistries, including HRP-conjugated secondaries, fluorophore labeling, or biotin–streptavidin systems for both chromogenic and fluorescence imaging on fixed tissue sections. Because the product is polyclonal, it offers epitope-level redundancy that is useful when probing receptor variants in denaturing gels or across heterogeneous receptor populations in clinical samples.

For western blot, researchers commonly resolve 20–40 µg of whole-cell lysate per lane; for IHC on paraffin-embedded tissue, working dilutions should be titrated against matched control tissue to optimize signal-to-noise. Aliquot upon receipt and store refrigerated between uses to preserve activity across freeze-thaw cycles. The 100 µl volume supports multiple immunoblotting and staining runs depending on membrane size, section count, and detection sensitivity.

Reactivity is reported for human GR (also known as NR3C1), making this antibody well suited to studies of stress response pathways, glucocorticoid pharmacology, inflammatory signaling, and hormone-driven transcriptional regulation in cell and tissue models.