Anti-GPR19 Antibody

Primary antibody reagents against orphan G protein-coupled receptors support mechanistic studies of under-characterized signaling pathways. This unconjugated rabbit polyclonal reagent binds the GPR19 receptor, an orphan GPCR implicated in melanoma cell proliferation, central nervous system expression patterns, and emerging metabolic regulation research. Antibodies.com validates the reagent for Western blot and immunocytochemistry workflows, and reactivity is confirmed across human, mouse, and rat samples, enabling cross-species translational studies in cell lines and primary tissues.

The formulation contains 1 mg/ml antibody in phosphate buffered saline (PBS) supplemented with 0.05% sodium azide, adjusted to approximately pH 7.2, and is supplied in a 100 µl vial sized for multiple membrane and imaging experiments. The polyclonal preparation broadens epitope coverage relative to monoclonal alternatives, providing better tolerance for minor sequence variation between species. Sodium azide acts as a preservative, so laboratories planning live-cell staining or downstream conjugation should perform a buffer exchange before use.

Investigators apply this antibody to map GPR19 distribution in melanoma lines such as A375, in rodent brain sections, and in peripheral tissues where the receptor transcript has been detected. Refrigerate the vial on arrival, protect it from light during storage, and prepare single-use aliquots to limit freeze-thaw cycles and preserve binding activity throughout the recommended shelf life.