Anti-GPR143 Antibody

Raised in rabbit and purified to an estimated purity of greater than 95%, this polyclonal IgG reagent from Antibodies.com is directed against GPR143, the G-protein coupled receptor also known as ocular albinism type 1 protein. The unconjugated antibody is supplied as a 50 µL liquid at 1 mg/mL in phosphate buffered saline (PBS) supplemented with 15 mM sodium azide at approximately pH 7.2, and is recommended for Western blot applications. The antibody has been validated for reactivity with human, mouse and rat samples, making it a useful tool for cross-species expression studies of this seven-transmembrane-domain melanocyte and eye-enriched receptor. As a research-use-only reagent, the product is shipped under standard cold-chain conditions and should be handled according to typical sodium azide-containing buffer precautions. Recommended WB working dilutions are generally in the 1:500 to 1:2000 range, and the polyclonal format ensures recognition of multiple epitopes across the GPR143 sequence. The unconjugated format offers flexibility for downstream labelling with HRP, biotin, fluorophore or other reporter conjugates of the user's choice, and the high purity makes it suitable for both standard immunoblotting and more demanding applications such as immunoprecipitation. For best stability, aliquot and store at -20 °C and avoid repeated freeze–thaw cycles. Researchers investigating ocular albinism, melanosome biology, or G-protein coupled receptor signalling in pigment cells will find this Antibodies.com reagent a reliable, well-characterized option for Western blot detection of GPR143.