Anti-GPLD1 Antibody

Supplied as 100 µl of unconjugated antiserum raised in rabbit, this reagent detects human glycosylphosphatidylinositol-specific phospholipase D1 (GPLD1) in western blot and immunofluorescence workflows. The polyclonal response against multiple epitopes on the human protein supports consistent detection across varied sample preparations, including cell and tissue lysates probed by western blot and fixed specimens examined by immunofluorescence. Because the antibody is unconjugated, laboratories pair it with the HRP-, fluorophore-, or biotin-conjugated secondary of their choice, which keeps multiplex or singleplex labeling flexible. Host species, clonality, and reactivity reported as human guide the secondary-reagent selection and experimental controls.

Available through Antibodies.com, GPLD1 is a liver-enriched enzyme that hydrolyzes glycosylphosphatidylinositol anchors and releases GPI-tethered proteins such as alkaline phosphatase and decay-accelerating factor from the plasma membrane, so this antibody is relevant to studies of GPI-anchor remodeling, hepatic secretory function, lipoprotein metabolism, and the cellular distribution of GPI-anchored receptors. Researchers working on in vitro membrane biology or cell-surface proteome turnover find it a practical probe for both abundance and localization. Aliquot upon receipt, store the vial refrigerated, and protect from light to preserve activity through the stated shelf life; consult the lot-specific data sheet for recommended starting dilutions and incubation times for western blot and immunofluorescence protocols.