Anti-GPD2 Antibody

Affinity-Purified Reagent for Glycerol-3-Phosphate Dehydrogenase 2

This unconjugated rabbit polyclonal antibody from Antibodies.com recognizes the mitochondrial isoform of glycerol-3-phosphate dehydrogenase (GPD2), the flavoenzyme that anchors the glycerophosphate shuttle linking cytosolic NADH to the electron transport chain at the outer surface of the inner mitochondrial membrane.

Characterized for both Western blot and immunohistochemistry on human, mouse, and rat specimens, the reagent supports comparative studies of hepatic gluconeogenesis, pancreatic beta-cell redox balance, and metabolic reprogramming in tumor cells that depend on glycerophosphate-driven respiration. Published applications include mapping altered shuttle flux in obesity and type 2 diabetes models, and localizing GPD2 within mitochondria of cancer cell lines maintained under hypoxic stress.

Supplied as 100 µl of liquid stock at 1 mg/ml in PBS containing 0.1% sodium azide and 50% glycerol, the antibody is ready to dilute into working buffer. Typical concentrations fall in the 1-5 µg/ml range for Western blot overnight incubation at 4 °C, and 5-10 µg/ml for IHC on paraffin-embedded tissue with standard antigen retrieval, producing the expected cytoplasmic and granular mitochondrial staining pattern on fixed sections.

Store the vial at -20 °C in single-use aliquots to limit freeze-thaw damage and keep working solutions refrigerated at 4 °C, protected from light. A research use only product from Antibodies.com, intended for laboratories investigating mitochondrial bioenergetics, NADH shuttling, and the cell biology of glucose and lipid metabolism.