Anti-GPC3 Antibody

From Antibodies.com, this is a rabbit polyclonal antibody directed against Glypican 3 (GPC3), supplied unconjugated in IgG form. The antibody was raised against a synthetic peptide corresponding to amino acids 481–580 of human Glypican 3 (NP_004475.1), with the target protein reported at a molecular weight of 66 kDa.

It is reactive with human, mouse, and rat samples. Listed applications include western blot, with the Antibodies.com catalogue additionally listing immunofluorescence and the manufacturer's documentation listing flow cytometry. The product is supplied as a liquid in Phosphate Buffered Saline (pH 7.3) containing 50% glycerol and 0.05% Proclin 300, purified by affinity methods.

Recommended dilutions are 1:500–1:1,000 for western blot and 1:50–1:200 for flow cytometry. The antibody is shipped at 4°C, aliquoted and stored at –20°C upon receipt, with freeze/thaw cycles avoided.