Anti-GOT2 Antibody

The rabbit polyclonal Anti-GOT2 reagent from Antibodies.com targets mitochondrial aspartate aminotransferase (GOT2), an enzyme anchored at the inner mitochondrial membrane where it participates in the malate-aspartate shuttle and supports NADH/NAD+ balance between cytosol and matrix. This unconjugated IgG preparation, supplied at 200 µl per vial, is validated for western blot and flow cytometry on human samples and is intended for research use only, allowing investigators flexibility to pair it with fluorescent, enzymatic, or biotin-based secondary detection systems.

GOT2 has attracted attention in studies of pancreatic ductal adenocarcinoma, breast cancer metabolism, and hepatic gluconeogenesis, where the enzyme's role in redox homeostasis and aspartate-driven nucleotide synthesis influences tumor cell proliferation and stress responses. The polyclonal format offers broader epitope coverage than a monoclonal alternative, which can be useful when probing denatured targets on membranes or fixed cells in flow cytometry. Researchers should aliquot on receipt to limit freeze-thaw cycles, protect the preparation from light if used downstream of a conjugation step, and follow lot-specific validation data for optimal dilution in their particular assay.