Anti-GOT2 Antibody

Raised in rabbit against the mitochondrial enzyme Glutamic-Oxaloacetic Transaminase 2 (GOT2), this unconjugated polyclonal antibody from Antibodies.com is supplied as 100 µl of liquid at 1 mg/ml in PBS containing 0.1% sodium azide and 50% glycerol, a formulation that protects the immunoglobulin during freezer storage at -20 °C. GOT2 catalyzes the reversible transamination between oxaloacetate and glutamate and is a core component of the malate-aspartate shuttle that ferries reducing equivalents across the inner mitochondrial membrane, making it a frequent subject of research in cancer metabolism, mitochondrial function, and hepatic or cardiac bioenergetics.

The reagent is validated for western blot, immunohistochemistry, immunofluorescence, and flow cytometry on human, mouse, and rat samples, so the same vial can support protein expression surveys in cell lysates, fixed tissue sections, adherent cultured cells, and suspension populations. The unconjugated format leaves the choice of secondary detection chemistry, fluorophore, or enzyme to the end user, and the rabbit host pairs with most commercially available anti-rabbit secondaries. Aliquoting upon first thaw is recommended to limit repeated freeze-thaw cycles of the glycerol stock.