Anti-GNPDA2 Antibody

Raised in rabbits against an 8 amino acid synthetic peptide near the C-terminus of human GNPDA2, this unconjugated polyclonal IgG reagent from Antibodies.com is affinity-purified and validated for Western blot detection of glucosamine-6-phosphate deaminase 2 in human and mouse samples. GNPDA2 is the second mammalian glucosamine-6-phosphate isomerase; it catalyzes the reversible conversion of D-glucosamine-6-phosphate into D-fructose-6-phosphate and ammonium, placing the antibody in workflows that probe hexosamine pathway activity, comparative glucosamine metabolism, and obesity-associated GWAS loci where GNPDA2 variants have been linked to BMI.

For Western blot, a working concentration near 1 µg/mL is suggested, with human kidney lysate serving as a positive control and an expected band at approximately 31 kDa corresponding to the mature isoform. Because the reagent is unconjugated, it pairs with a user-selected HRP, fluorescent, or alkaline phosphatase secondary antibody, preserving flexibility for chemiluminescent or multiplex readouts. Aliquot upon receipt and store at -20°C to limit freeze-thaw loss of titer. The antibody is predicted not to cross-react with the paralog GNPDA1 and is intended for in vitro research use only.