Anti-GNAQ Antibody

Raised in rabbit against the GNAQ target, this unconjugated polyclonal reagent from Antibodies.com is supplied as 100 µl of IgG and is validated for Western blot (WB) detection of human samples. GNAQ encodes the Gαq subunit of a heterotrimeric G protein that couples G-protein-coupled receptors to phospholipase C-β, mobilizing intracellular calcium and activating protein kinase C downstream of extracellular ligand binding. Researchers studying melanocyte signalling, uveal melanoma biology, blue nevi, and vascular anomalies linked to somatic GNAQ alterations (such as Sturge-Weber syndrome and port-wine stains) routinely probe total GNAQ levels in cell lysates by immunoblotting, where the polyclonal profile helps capture multiple epitopes and improves recognition of denatured protein.

With a rabbit IgG isotype and no conjugated tag, the reagent supports secondary-antibody workflows including chemiluminescent Western blot detection and pairs with standard HRP- or fluorophore-labelled anti-rabbit secondaries. For repeated use, dispense into single-use aliquots and refrigerate; brief freeze-thaw cycles are tolerated but repeated cycling should be avoided to preserve binding activity. The 100 µl pack size is sufficient for multiple mini- or midi-gel experiments at typical working dilutions, and the unconjugated format leaves the choice of reporter label to the experimental design.