Anti-GNAO1 Antibody

A rabbit-derived polyclonal reagent from Antibodies.com that recognizes the GNAO1 protein, the alpha subunit of the heterotrimeric G protein Go that couples receptors to downstream effectors in neuronal membranes. The antibody is supplied at 1 mg/ml in PBS with 0.1% sodium azide and 50% glycerol as an unconjugated 100 µl aliquot, prepared for direct labeling or use in standard detection workflows. It has been validated for Western blot and immunohistochemistry and was raised against epitopes conserved across human, mouse, and rat GNAO1, supporting cross-species studies of G protein signaling in brain tissue and heterologous expression models.

GNAO1 is broadly expressed in the central nervous system, where it modulates voltage-gated calcium channels and contributes to inhibitory regulation of neurotransmitter release. Loss-of-function and gain-of-function mutations in GNAO1 are linked to a spectrum of neurodevelopmental disorders, including early infantile epileptic encephalopathy type 17 (EIEE17) and a movement disorder syndrome featuring chorea, dystonia, and dyskinesia. Researchers studying these conditions rely on robust detection of endogenous GNAO1 in patient-derived cells, knock-in rodent models, and post-mortem tissue. The unconjugated format allows researchers to pair this reagent with HRP, biotin, or fluorophore conjugates of their choice, and the glycerol-containing buffer is compatible with storage at -20°C with minimal freeze-thaw degradation.

Recommended applications include Western blot detection of the ~40 kDa GNAO1 band in cell and tissue lysates, and immunohistochemistry on formalin-fixed paraffin-embedded brain sections or fresh-frozen tissue from rodent models. Each lot is affinity-purified and supplied with validation data on the product page. For research use only; not for diagnostic or therapeutic procedures.