Anti-Glucagon Antibody

A rabbit polyclonal antibody from Antibodies.com raised against the pancreatic peptide hormone glucagon, supplied as 50 µl of unconjugated antiserum for use in Western blot and immunofluorescence workflows across human, mouse, and rat samples.

Glucagon is a 29-amino acid counter-regulatory hormone secreted by pancreatic alpha cells that drives hepatic glucose output. Reliable detection of this proglucagon-derived peptide is a routine requirement in studies of islet architecture, beta-cell mass, alpha-cell differentiation, and metabolic disease models. The polyclonal format presents multiple epitope specificities on the glucagon precursor, which can be advantageous when probing tissue sections or cell preparations where accessibility to any single linear epitope is limited.

Recommended applications include Western blot for resolving proglucagon and mature peptide forms in cell lysates or tissue extracts, and immunofluorescence on frozen or fixed pancreatic sections to localize alpha cells within islets. The reagent is intended for research use only, in vitro labeling experiments. Aliquot upon first use to limit freeze-thaw cycles, and store refrigerated for working aliquots or frozen for longer-term storage, protecting the vial from light to preserve antibody activity.