Anti-GLS Antibody

Glutaminase (GLS) catalyzes the hydrolysis of glutamine to glutamate and ammonia, a reaction central to cellular nitrogen metabolism, TCA cycle anaplerosis, and the redox balance that proliferating cells rely on. This unconjugated rabbit polyclonal reagent from Antibodies.com is raised against the GLS target and supplied as 100 µl of antiserum for Western blot and immunohistochemistry workflows on human samples.

The polyclonal format presents an epitope pool that tolerates minor denaturation-induced conformational changes during SDS-PAGE, an advantage when probing cleavage products of full-length kidney-type glutaminase (~65 kDa) or the shorter GAC splice variant (~58 kDa). Rabbit IgG supports chromogenic and fluorescent detection strategies without species conflicts in commonly used secondary reagents, and unconjugated chemistry lets the end user select HRP, biotin, or fluorophore conjugates downstream.

Recommended storage is refrigerated at 4 °C in working aliquots to limit freeze-thaw degradation of the IgG preparation. Each vial is intended for single-use sessions; researchers commonly pair this reagent with cell lysate inputs of 20–50 µg total protein per lane for clean chemiluminescent Western blot signals, and with citrate-buffer antigen retrieval for IHC on formalin-fixed, paraffin-embedded tissue.