Anti-GJC2 Antibody

Connexin 47 (Cx47), the protein product of the GJC2 gene, forms hexameric connexons that assemble into transmembrane gap junction channels. These channels facilitate the passage of ions, second messengers, and small metabolites between adjacent cells, playing an essential role in maintaining cellular homeostasis. Expressed primarily by myelinating oligodendrocytes in the central nervous system and by Schwann cells in the peripheral nervous system, Cx47 localizes to myelin sheaths, paranodal loops, and Schmidt-Lanterman incisures. Mutations in this gene are directly linked to hereditary leukoencephalopathies, including Pelizaeus-Merzbacher-like disease 1 (PMLD1), hereditary spastic paraplegia 44 (SPG44), and lymphedema. Affinity purified from rabbit hosts, this polyclonal IgG reagent from Antibodies.com detects endogenous levels of total GJC2 across human, mouse, and rat samples. Researchers apply this unconjugated primary antibody in western blot (WB) and immunohistochemistry (IHC) protocols to investigate glial cell communication, demyelination, and potassium siphoning. The standard vial format provides flexibility for both chromogenic and immunofluorescence workflows when paired with appropriate secondary antibodies. Supplied as a 50 µl liquid volume, the reagent should be stored refrigerated and protected from light. To maintain the shelf life and preserve antibody activity, prepare single-use aliquots to minimize freeze-thaw cycles.