Anti-GJB6 Antibody

Connexin 30 (Cx30) is the protein product of the GJB6 gene, a four-pass transmembrane gap junction subunit assembled into connexon hemichannels that mediate direct intercellular signaling in epithelial, neural, and cochlear tissues. This rabbit polyclonal IgG antibody from Antibodies.com binds Cx30 and is supplied as an unconjugated 100 µl vial of liquid stock validated for Western blot application on human samples, making it a useful reagent for researchers studying connexin trafficking, gap junction plaque assembly, and tissue-specific channel function. Loss-of-function mutations in GJB6 underlie autosomal recessive nonsyndromic hearing loss and the hidrotic ectodermal dysplasia Clouston syndrome, two established genotype-phenotype associations widely investigated in auditory and dermatologic research where Cx30 expression in the inner ear sensory epithelium and the epidermal layers of the skin is most relevant. The polyclonal format presents multiple epitopes across the Cx30 sequence, supporting consistent detection in immunoblotting workflows where the target migrates near its predicted molecular weight. Aliquotting on first use is recommended to limit freeze-thaw cycles; refrigeration is fine for short-term handling, while long-term storage at -20 °C in working aliquots preserves activity. Researchers investigating cochlear gap junction networks, epidermal barrier proteins, or the genetic basis of hereditary hearing impairment can pair this reagent with appropriate secondary antibodies and matched isotype controls to generate reproducible Western blot signals from human cell and tissue lysates.