Anti-GJA1 (phospho S368) Antibody

Target Connexin 43 (GJA1) phosphorylated at serine 368 with this rabbit polyclonal IgG antibody from Antibodies.com. The reagent is supplied as 200 µl of unconjugated antiserum and is validated for Western blot and immunohistochemistry applications. It detects the phosphorylated form of human, mouse, and rat GJA1, enabling studies of gap junction regulation in cardiac, neural, and epithelial tissue models.

Phosphorylation at Ser368 is a well-characterized post-translational modification that controls gap junction assembly, internalization, and channel gating. Researchers use this reagent to investigate how kinase signaling cascades — including PKC and MAPK pathways — modulate connexin function during development, wound repair, ischemic injury, and tumor progression. The polyclonal format provides recognition of multiple epitopes surrounding the phospho-S368 site, supporting signal in Western blot and IHC workflows on paraffin and frozen tissue sections.

Each aliquot is provided as a liquid for handling flexibility. The IgG isotype allows pairing with standard anti-rabbit secondary reagents. Researchers performing time-course stimulation studies, such as phorbol ester or growth factor treatments, can track dynamic changes in connexin phosphorylation status alongside total GJA1 levels.