Anti-GIRK1 Antibody

Produced in rabbit and affinity-purified for research use, this Antibodies.com polyclonal reagent targets the KCNJ3-encoded G protein-activated inward rectifier potassium channel subunit (GIRK1). The unconjugated antibody is supplied as 100 µL of working stock and is validated for Western blot and immunohistochemistry on human samples, supporting studies of inhibitory G-protein signaling in cardiac and neuronal tissues.

GIRK1 assembles with Kir3 family partners to form heterotetrameric channels gated by Gβγ subunits released from Gi/o-coupled receptors. Investigators studying opioid and reward circuitry, cardiac pacemaking, and slow inhibitory postsynaptic potentials depend on reproducible immunolabeling across brain regions and disease models. The polyclonal format offers multiple epitope coverage per target, useful when monoclonal reagents fail to detect conformationally masked variants.

Suggested starting dilutions vary by application and sample type, and researchers should titrate against positive-control lysates or fixed tissue to determine optimal signal-to-noise. Aliquot upon receipt and store the vial at -20°C, avoiding repeated freeze-thaw cycles to preserve antigen-binding activity. Refer to the lot-specific data sheet shipped with the reagent for buffer composition and any updated handling notes.