Anti-GFP Tag Antibody

This rabbit polyclonal IgG antibody targets GFP Tag and is provided unconjugated by Antibodies.com. It was raised against a recombinant fusion protein containing the sequence corresponding to amino acids 1–238 of GFP, a target protein of approximately 25 kDa. The reagent is affinity purified and supplied as a liquid in Phosphate Buffered Saline (pH 7.3) with 50% glycerol and 0.01% thiomersal.

Manufacturer-recommended working dilutions are 1:2,000–1:20,000 for Western blot and 1:50–1:200 for immunocytochemistry/immunofluorescence (ICC/IF). The product is shipped at 4°C and on arrival should be aliquoted and stored at −20°C, with freeze/thaw cycles avoided.

Validated applications include Western blot and ICC/IF, with immunohistochemistry also listed among the supported uses.