Anti-GFP Tag Antibody

Raised in mouse hosts and purified as a polyclonal IgG preparation, this primary antibody from Antibodies.com targets Green Fluorescent Protein and its commonly used spectral variants expressed as fusion tags on recombinant proteins. The polyclonal format binds multiple epitopes across the GFP β-barrel structure, increasing the likelihood of recognizing partially denatured or truncated GFP-tagged constructs in routine expression analysis.

Supplied unconjugated at a volume of 50 µl, the reagent is validated for western blot, immunofluorescence, and immunoprecipitation workflows. The unconjugated format gives researchers flexibility to pair it with their preferred secondary antibody, fluorescent dye, or enzyme reporter depending on the application. It performs reliably when probing lysates or fixed samples from transfected cells expressing GFP, EGFP, or related fluorescent protein fusions.

Polyclonal preparations generally yield stronger signals than monoclonal alternatives on western blots because they target several distinct antigenic sites simultaneously. For immunofluorescence, the antibody enables direct visualization of GFP fusion protein localization in cultured cells and tissue sections, while the immunoprecipitation compatibility supports efficient pull-down of GFP-tagged complexes for interaction studies and co-immunoprecipitation experiments.

For short-term use, store the antibody refrigerated, then aliquot into smaller working volumes before freezing at -20°C for long-term storage to limit freeze-thaw cycles that can compromise IgG binding activity over time.