Anti-GFP Antibody [D4-18]

The Anti-GFP Antibody [D4-18] from Antibodies.com is a mouse monoclonal IgG1 reagent supplied as a 100 ug liquid aliquot in a single-use vial. Clone D4-18 was raised against full-length Green Fluorescent Protein and recognizes native GFP (~27 kDa) as well as common spectral variants such as EGFP, providing broad cross-reactivity across GFP-tagged constructs expressed in mammalian cells. Validated for Western blot and immunoprecipitation, the unconjugated format enables direct detection of GFP fusion proteins in cell lysates and recovered immunoprecipitates without the need for a secondary label. Researchers commonly use this reagent to verify expression of GFP-tagged constructs, co-immunoprecipitate GFP-tagged interaction partners, and track tagged proteins across biochemical purification workflows. For long-term storage, freeze the antibody at -20 degrees C in working aliquots and limit freeze-thaw cycles to preserve binding activity and lot-to-lot consistency.