Anti-GFP Antibody

Affinity reagents for fluorescent protein studies are offered by Antibodies.com within its Primary Antibodies range. This unconjugated reagent is a Rabbit polyclonal antibody to GFP, raised in rabbit as a polyclonal IgG and reactive species-independently. It is supplied as a liquid.

The antibody was raised against recombinant full-length Green Fluorescent Protein from Aequorea victoria and is reactive against all variants of Aequorea victoria GFP such as S65T-GFP, RS-GFP, YFP, CFP, RFP, and EGFP. The supplier lists applications of WB, ELISA, ICC, IHC-Fr, IHC-P, IP, IHC-FoFr, IHC-FrFl, and Electron Microscopy, while the manufacturer additionally validates Flow Cytometry. Seven scientific validation images are referenced.

The product is prepared as whole antiserum and supplied as an aliquot of serum containing 0.05% Sodium Azide and 1.25% Sodium Chloride. It is shipped at 4°C and should be aliquoted and stored at -20°C or -80°C upon delivery, avoiding freeze/thaw cycles. Recommended dilutions are WB 1:1,000-1:2,500, ICC 1:200-1:1,000, Flow Cytometry 1:100, IHC-P 1:500-1:1,000, IHC-Fr 1:500-1:2,000, and Electron Microscopy 1:1,000-1:4,000.