Anti-GFP Antibody

Rabbit polyclonal antibody to GFP, supplied as an unconjugated liquid primary antibody by Antibodies.com for use across a range of research techniques.

The reagent is raised in rabbit, is of the IgG isotype, and has been validated for Western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry (IHC). The immunogen is recombinant EGFP carrying a His tag, and the antibody reacts with multiple variants of Aequorea victoria GFP, including S65T-GFP, RS-GFP, YFP, and EGFP, as well as their fusion proteins.

It is supplied as antiserum containing 0.05% sodium azide and shipped at 4°C; upon delivery it should be aliquoted and stored at -20°C, with freeze/thaw cycles avoided. Recommended dilutions are WB 1:2,000, IF 1:4,000, and IHC 1:4,000. The antibody has been cited in 1 publication and is supported by 4 scientific validation images.