Anti-GFAP Antibody - Identical to Sigma (SAB4300647)

This Rabbit polyclonal antibody to GFAP is offered by Antibodies.com as part of their Primary Antibodies portfolio, formulated to detect the endogenous level of total GFAP protein in validated Western blot (WB) and immunocytochemistry/immunofluorescence (ICC/IF) workflows. Supplied at 1.0 mg/mL in a 50 µl volume, the reagent is unconjugated and raised in Rabbit against an immunogen corresponding to the peptide sequence around amino acids 423–427 (E-S-K-Q-E) of human GFAP. Antibodies were produced by immunizing rabbits with a synthetic peptide–KLH conjugate and subsequently purified by affinity chromatography using the epitope-specific peptide. The immunoglobulin isotype is IgG, the host is Rabbit, and the clonality is Polyclonal, making this reagent appropriate for studies of glial fibrillary acidic protein, a class-III intermediate filament that serves as a cell-specific marker distinguishing astrocytes from other glial cells in the central nervous system. Reactivity has been confirmed in Human and Rat samples, consistent with published work on the equivalent Sigma reference SAB4300647. The product is provided in phosphate-buffered saline (without Mg²⁺ and Ca²⁺) at pH 7.4, containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol, and is shipped at 4 °C; upon receipt it should be aliquoted and stored at –20 °C, with repeated freeze/thaw cycles avoided to preserve activity. For research use only.