Anti-GDF-15 Antibody

Raised in rabbit against the human Growth Differentiation Factor 15 (GDF-15) protein, this unconjugated polyclonal antibody is supplied by Antibodies.com as a 100 µl vial of ready-to-use reagent at 1 mg/ml in PBS containing 0.1% sodium azide and 50% glycerol as a cryoprotectant. The reagent is listed for Western blot and immunohistochemistry workflows, and it cross-reacts with both human and rat samples, making it useful for translational studies that compare expression in patient-derived tissues with rodent disease models.

GDF-15 is a divergent member of the transforming growth factor-β superfamily and a stress-inducible cytokine whose expression rises in response to mitochondrial dysfunction, inflammation, and cellular senescence. Researchers apply this reagent to probe GDF-15 induction in cancer cachexia models, cardiovascular risk stratification, and investigations of the GDF15–GFRAL axis that controls appetite and energy homeostasis. The glycerol-containing buffer permits storage at -20°C with minimal freeze-thaw damage, while sodium azide acts as a microbial preservative for the working dilution; protect from light during handling.

For Western blot, the antibody is applied to whole-cell or tissue lysates under reducing conditions to detect the mature secreted dimer. For immunohistochemistry on formalin-fixed paraffin-embedded sections, antigen retrieval with citrate buffer at pH 6.0 is recommended before overnight incubation. End users should titrate the antibody empirically for their specific tissue or cell system. For research use only, not for diagnostic or therapeutic applications.