Anti-GCNT1 Antibody

GCNT1 (glucosaminyl N-acetyltransferase 1, also known as C2GNT) is the Golgi-resident β-1,6-N-acetylglucosaminyltransferase that initiates core 2 branching on mucin-type O-glycans by transferring GlcNAc onto GalNAc-α-Ser/Thr. This rabbit polyclonal IgG preparation from Antibodies.com is supplied as a 200 µL aliquot of unconjugated antiserum and is validated for Western blot detection of the human protein.

The antibody is used in glycosylation research, leukocyte-trafficking studies, and oncology workflows. GCNT1-derived core 2 structures decorate P-selectin glycoprotein ligand-1 and govern selectin-mediated rolling, while altered GCNT1 expression reshapes the mucin glycocalyx of breast and colon carcinoma cells and modulates E-cadherin–dependent adhesion and metastatic spread. Researchers apply this reagent to confirm expression in cell lines and tumor lysates, validate CRISPR or RNAi knockdowns, and probe how shifts in O-glycan branching restructure the cell surface.

Reagent characteristics: host Rabbit, clonality polyclonal, isotype IgG, conjugate unconjugated, reactivity against human samples, recommended application WB. Store refrigerated; protect from light and aliquot to limit freeze-thaw cycles of the polyclonal solution. The 200 µL volume supports multiple probing experiments and accompanying loading-control blots.