Anti-GCLM Antibody

Manufactured by Antibodies.com, this rabbit polyclonal reagent is raised against recombinant human GCLM (glutamate-cysteine ligase modifier subunit) and affinity-purified using the immunogen to ensure selective recognition of the endogenous target. Provided as an unconjugated IgG at 1.0 mg/mL in a 50 µL volume, the antibody is formulated in phosphate-buffered saline at pH 7.4 with 150 mM NaCl, 0.02% sodium azide, and 50% glycerol as a cryoprotectant. It is shipped at 4 °C and should be aliquoted for long-term storage at −20 °C, with freeze/thaw cycles avoided to preserve activity. Validated applications include Western blotting (WB), immunohistochemistry (IHC), and immunocytochemistry/immunofluorescence (ICC/IF), making it a flexible tool for studies of redox homeostasis and glutathione biosynthesis across multiple model systems. Demonstrated reactivity covers human, mouse, and rat samples, enabling comparative work between cell lines and primary tissues. The polyclonal format increases the likelihood of robust signal even when individual epitopes are altered, and the unconjugated presentation allows researchers to couple it to their preferred fluorophore or enzyme depending on the downstream detection method.