Anti-GCLM Antibody

Raised in rabbit and supplied as 100 µl of unconjugated antisera, this polyclonal reagent from Antibodies.com targets the modifier subunit of glutamate-cysteine ligase (GCLM) for use in western blotting, immunohistochemistry, and immunofluorescence workflows. GCLM is the regulatory subunit of the rate-limiting enzyme in de novo glutathione biosynthesis, and the antibody recognizes the endogenous human, mouse, and rat protein.

This reagent supports studies of cellular redox balance, oxidative-stress responses, and detoxification pathways, where GCLM expression is typically examined alongside the catalytic GCLC subunit. The unconjugated format lets researchers pair the antibody with their preferred fluorophore or enzyme conjugate for custom detection, and the polyclonal nature provides broad epitope coverage useful when probing samples with limited material or variable expression levels.

Investigators in hepatic glutathione metabolism, neurodegeneration models, and tumor redox biology have used GCLM antibodies to track changes in holoenzyme assembly and activity under conditions such as xenobiotic challenge, Nrf2 activation, or ferroptosis induction. Each vial is intended for laboratory research use only.