Anti-GCK Antibody

Glucokinase (GCK), also known as hexokinase IV, serves as a critical glucose-sensing enzyme in hepatocytes and pancreatic beta cells, where it catalyzes the ATP-dependent phosphorylation of glucose to glucose-6-phosphate. This rabbit polyclonal reagent from Antibodies.com is raised against GCK and validated for Western blot and immunofluorescence workflows across human, mouse, and rat samples.

Researchers investigating glucose homeostasis, hepatic insulin signaling, and the molecular basis of Maturity Onset Diabetes of the Young (MODY type 2) rely on anti-GCK antibodies to map expression patterns and track enzyme levels in tissue and cell-based models. The unconjugated format allows flexible pairing with secondary detection reagents, including HRP- or fluorophore-conjugated anti-rabbit IgGs, depending on the experimental read-out.

Supplied as a 100 µl aliquot in a vial, the antibody supports detection of GCK from tissue lysates and visualization of subcellular distribution by immunofluorescence in cultured cells or cryosections. Polyclonal reactivity against multiple epitopes aids recognition even when minor conformational changes or post-translational modifications are present in the target protein.