Anti-GATA4 (Q258) Antibody

This unconjugated polyclonal primary antibody from Antibodies.com targets GATA4, a zinc-finger transcription factor essential for cardiac morphogenesis, cardiomyocyte specification, and gonadal development. Raised in rabbit against the Q258 epitope, the reagent is validated for immunohistochemistry (IHC) on formalin-fixed paraffin-embedded (FFPE) and frozen tissue sections from human, mouse, and rat samples.

Supplied as 50 µl of antibody at 1 mg/ml in phosphate buffered saline (PBS) with 0.05% sodium azide at approximately pH 7.2, the vial accommodates standard immunostaining workflows. Researchers investigating congenital heart defects, sex determination pathways, endoderm-derived organogenesis, and germ cell development rely on this antibody to map expression patterns in embryonic and adult tissues. The reagent tolerates common antigen retrieval methods, including citrate- and EDTA-based heat-induced protocols, and supports localization of endogenous GATA4 protein in cardiomyocytes, gonadal somatic cells, and hepatic tissue.

Refrigerate upon arrival; protect from light, avoid repeated freeze-thaw cycles, and aliquot if extended storage is needed to preserve long-term activity. Applications extend to studies of cardiac hypertrophy and gastric cancer research, where GATA4 expression serves as a clinically relevant marker of tumor differentiation and prognosis.