Anti-GAPDH Antibody

This unconjugated primary antibody from Antibodies.com targets glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a constitutively expressed 37 kDa glycolytic enzyme routinely used as a loading control in immunoblotting workflows. Raised in rabbit and supplied as a polyclonal reagent in a 100 µL vial format, it is validated for Western blot, immunohistochemistry, and ELISA applications across human, mouse, and rat sample types.

Because GAPDH is highly and stably expressed in most tissues, the antibody supports normalization of target band intensity in Western blot loading studies and serves as a reliable cytoplasmic marker in IHC staining of formaldehyde-fixed paraffin-embedded tissue sections. The unconjugated format offers researchers flexibility to pair the reagent with their preferred HRP- or fluorophore-conjugated secondary antibody, which proves useful when designing multiplex fluorescence experiments or when customizing detection chemistries for cell-based assays.

For storage, working aliquots should be frozen at -20°C for long-term stability, with diluted reagent stored refrigerated and protected from light to preserve performance across freeze-thaw cycles. The reactivity profile covers Human, Mouse, and Rat GAPDH, allowing direct comparison of housekeeping-protein expression between cell lines and primary tissue samples from these three species on the same blot or slide.