Anti-GAPDH Antibody

Produced by Antibodies.com, this rabbit polyclonal reagent recognizes glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a constitutively expressed housekeeping enzyme routinely used as a loading-control target in Western blot workflows. The antibody is supplied as an unconjugated IgG preparation at 1 mg/ml in a 100 µl vial, formulated in phosphate buffered saline (PBS) with 0.05% sodium azide at approximately pH 7.2 to maintain reagent stability. Validated for WB applications, it cross-reacts with GAPDH from human, mouse, and rat samples and is broadly reactive across mammalian species, supporting consistent lane-to-lane normalization in cell lysates and tissue homogenates prepared from diverse model systems. The polyclonal format increases the probability of detecting the target even when individual epitopes are masked by denaturation or reduction conditions, and the unconjugated format leaves selection of the secondary antibody, fluorophore, or enzyme reporter entirely to the end user. For routine handling, refrigerate the vial and protect from light between uses; the sodium azide included in the buffer helps control microbial growth across repeated freeze-thaw cycles when small aliquots are prepared from the stock. This primary antibody addresses a core requirement in biochemistry, molecular biology, and cell signalling studies, where quantitative comparison of band intensities depends on a reliable reference signal that remains stable across experimental conditions, treatment groups, and time points.