Anti-GAPDH Antibody

This Antibodies.com monoclonal antibody recognizes glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a highly conserved ~37 kDa housekeeping enzyme that participates in glycolysis and is widely deployed as an internal loading control across immunoblotting workflows. Supplied as 100 µg of unconjugated IgG1 in liquid format, the reagent is validated for Western blot, immunofluorescence, and ELISA applications on human and monkey samples. For best results, store the vial at -20 degrees C in single-use aliquots to limit freeze-thaw cycles, and avoid prolonged exposure to light. The antibody targets a cytosolic protein abundantly expressed in most tissues, producing a clean band near 37 kDa on reducing SDS-PAGE gels and a consistent cytoplasmic signal in fixed-cell imaging. Researchers commonly pair this clone with housekeeping-protein normalization when quantifying band intensity across treatment groups.