Anti-GAMT Antibody

GAMT (guanidinoacetate N-methyltransferase) is the terminal enzyme in the creatine biosynthesis pathway, catalyzing methylation of guanidinoacetate to creatine using S-adenosylmethionine as the methyl donor. This rabbit polyclonal reagent from Antibodies.com is raised against human GAMT and supplied as 100 µl of unconjugated antiserum in a single vial, ready for western blot (WB) and immunohistochemistry (IHC) workflows.

The antibody detects endogenous GAMT in human samples, supporting studies of hepatic creatine synthesis, neurological creatine deficiency syndromes (including guanidinoacetate methyltransferase deficiency), and broader investigations into cellular methyl group metabolism. Polyclonal reactivity allows recognition of multiple epitopes, which is helpful when working with lysates that may contain partial degradation products or post-translationally modified forms. The unconjugated format lets researchers couple the reagent to their preferred reporter, retaining full control over signal generation in chromogenic, fluorescent, or chemiluminescent detection systems.

For WB, the antibody can be paired with standard HRP-conjugated secondary reagents and chemiluminescent substrates. For IHC on formalin-fixed paraffin-embedded sections, antigen retrieval in citrate buffer (pH 6.0) followed by peroxidase or polymer-based detection is recommended. Short-term storage at 4 °C is appropriate for active use, while aliquoting and freezing at -20 °C extends shelf life across multiple experiments. Researchers studying inborn errors of creatine metabolism, muscle bioenergetics, hepatic function, or comparative methyltransferase biology will find this reagent useful for confirming GAMT expression in human cell lines, primary hepatocytes, and tissue specimens.